Genetic stability observed in third-generation progeny trial of Acacia mangium: the importance of genotype by environment interaction assessment in advance generation breeding strategy

Abstract

The breeding program for Acacia mangium has entered advanced-generation breeding cycles through adopting a recurrent selection system and a sub-lining breeding population. Genetic variation changes along the successive generations could affect its genetic stability on wide ranges of sites. The aim of this study is to observe genetic stability in third-generation progeny trials of A. mangium established at three different sites in Indonesia. Analysis was conducted, including single-site and multi-sites analyses for height, diameter, and stem forking that were grouped into two sets of analysis based on the genetic background of the trial: SET01 for the single sub-line and SET02 for the composite sub-lines. Index selection for multiple-traits was then used to identify the family changing ranks for multiple-traits and genetic gain prediction. The results showed that the recurrent selection system adopted in the breeding strategy for single-site analysis could maintain sufficient genetic variance of A. mangium in the third-generation progeny trial. Family heritability was moderate to high for almost all traits. However, a strong genetic-environment interaction (G × E) exists in multi-sites analysis for the single sub-line population (SET01), indicating a less sufficient genetic variation and a low Type B genetic correlation in anticipating a wider range of environment. On the contrary, compositing selected family from several sub-lines (SET02) could diminish the strength of G × E and increase Type B correlation. Selection and genetic gain prediction could be more effective in multi-sites analysis for SET02, but it was less effective for SET01. The results imply that adopting a recurrent selection system in advanced-generation breeding of A. mangium should consider structuring the breeding population. It could be practiced by compositing selected superior families from several sub-lines into one breeding population to maintain high genetic stability, while increasing genetic diversity and productivity.

Keywords:

• Recurrent selection system
• sub-lining system
• tree breeding
• genetic gain

Acknowledgements

We would like to express our deepest gratitude to The Centre for Forestry Instrument Standard Assessment, The Ministry of Environment and Forestry for providing the plots as the valuable resources in support of our research. Our thanks also go to the entire team involved in this research for their hard work and dedication in measurement and data entry. Thanks are due to Dr. Christopher Beadle, who kindly reviewed earlier drafts of the manuscript and suggested improvements.

Author contributions

Arif Nirsatmanto (A.N.), Sri Sunarti (S.S.), Asri Insiana Putri (A.I.P.), Liliek Haryjanto (L.H.), Noor Khomsah Kartikawati (N.K.K.), Toni Herawan (T.H.), Fajar Lestari (F.L.), Sugeng Pudjiono (S.P.) and Anto Rimbawanto (A.R.) contributed in conceptualization, methodology, software, validation, formal analysis, investigation, resources, data curation, writing—original draft preparation, writing-review and editing, visualization, supervision, and project administration. All authors had an equal role as main contributors in discussing the conceptual ideas and the outline, providing critical feedback for each section, and writing the manuscript. All authors have read and agreed to the published version of the manuscript

Disclosure statement

No potential conflict of interest was reported by the author(s).