Abstract
The effect of pH, carbon source and K+ on the Na+ -inhibited germ tube formation of the pathogenic fungus Candida albicans was examined in the arginine-phosphate modified (APM) medium. All C. albicans cells formed germ tubes in APM medium at pH 5·0-9·0. Na+ inhibited germ tube formation in a concentration dependent manner ranging from 0·2 to 1·0 M, and was further influenced by the pH of the medium. The inhibitory effect of Na+ was lowest at pH 8·0, and germ tube formation ceased at 1·0 M Na+ for any pH (4·0-9·0). At pH≥6·0, non-germ tube-forming cells did not show yeast growth; whereas at pH≤⃒5·0, Na+ inhibited only germ tube formation but did not inhibit yeast growth. The inhibitory effect of Na+ was stronger in glucose medium than in galactose medium as carbon source. K+, at 0-0·8 M, had almost no effect on germ tube formation. However, in the presence of Na+, a very low concentration of K+ (0·5 mM) was able to release the cells from Na+ arrest and produced an increase in the rate as well as the percentage of germ tube formation. Intracellular Na+/K+ ratios increased with the increase in extracellular Na+ concentration, whereas the ratios decreased and remained within nontoxic levels when the extracellular K+ concentration was increased.