Abstract
In order to determine the environmental occurrence of both varieties of Cryptococcus neoformans in the city of São Paulo, pigeon droppings and vegetable material from trees of the genus Eucalyptus were collected at typically crowded places. A total of 38 sites downtown where large heaps of pigeon droppings could be found were selected for sampling. Pigeon droppings from 10 (26·3%)of these sites were positive for C. neoformans var. neoformans in at least one sample. Twelve eucalyptus woods located within four municipal parks were also surveyed; vegetable material from Eucalyptus spp. trees were collected monthly over a 2-year period. C. neoformans var. gattii was recovered from a wood in Ibirapuera Park during the same season on two different occasions (November 1996 and November 1997); this park contained specimens of Eucalyptus camaldulensis, a natural habitat of C. n. var. gattii. C. n. var. neoformans was detected in a wood in Ibirapuera Park and Aclimação Park. The results show that both C. n. var. neoformans and C. n. var. gattii are present in the urban environment of São Paulo city at sites where large numbers of people normally gather.