Preparation and Characterization of Collagen–Hydroxyapatite Composite Used for Bone Tissue Engineering Scaffold

Abstract

In this study, highly porous collagen–HA scaffolds were prepared by solid–liquid phase separation method. Microstructure of the composites was characterized by SEM, TEM and XRD. The results show that collagen–HA scaffolds are porous with three‐dimension interconnected fiber microstructure, pore sizes are 50–150 µm, and HA particles are dispersed evenly among collagen fiber. Compared with pure collagen, the mechanical property of collagen–HA composite improves significantly. To gain further insight into cell growth throughout 3D scaffolds, the cell proliferation and attachment on the scaffold in vitro was investigated. The collagen–HA composite has good biocompatibility, and adding HA does not affect the histocompatibility of the scaffold materials. The porous collagen–HA composite is suitable as scaffold used for bone tissue engineering.

• Collagen–hydroxyapatite composite
• Bone tissue engineering
• Microstructure
• Histocompatibility