Ciprofloxacin‐Induced Glutathione Redox Status Alterations in Rat Tissues

Abstract

The possible oxidative stress inducing effect of a fluoroquinolone (FQ) antibiotic, ciprofloxacin (CPFX), was investigated in rats measuring glutathione redox status. For this purpose, the drug was administered to rats as two different single doses (100 and 150 mg/kg, ip) or a repeated dose (500 mg/kg/d, ig, for 5d). Then, total and oxidized glutathione levels were determined in hepatic and cerebral tissues of the rats by an enzymatic cycling assay, and the glutathione redox status was calculated. The possible protective effects of vitamin E or allopurinol against CPFX‐induced alterations on glutathione system have also been examined. Following both routes of administration of CPFX, the total glutathione content of the liver, but not of brain decreased significantly. The oxidized glutathione (GSSG) in the brain increased after single or repeated dose treatments, but only with repeated doses of CPFX in the liver. CPFX induced dose‐dependent alterations in the glutathione redox status in both tissues. With single doses the effect was more pronounced in cerebral tissue, and with repeated ig doses it was significant in both tissues. Pretreatment of rats with vitamin E or allopurinol before the administration of CPFX provided marked protection against glutathione redox status alterations in both tissues. Our results, thus, indicate that CPFX treatment introduces an oxidative stress in cerebral and hepatic tissues of rat.

Key Words: :

• Ciprofloxacin
• Glutathione redox status
• Vitamin E
• Allopurinol
• Oxidative stress

Acknowledgments

This study was supported by Hacettepe University Research Fund HÜAF 92‐03‐013. We would like to thank Dr. Tambay Taskin from Deva Laboratory for the generous gift of ciprofloxacin.

Notes

^aUnpublished results: Ciprofloxacin‐induced free radical generation in cerebral microsomes of rats. Aylin Gürbay, Brigitte Gonthier, Denis Daveloose, Alain Favier, Filiz Hıncal.