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Abstract
Leukotrienes (LTs) are recognized to be important mediators in asthma. Recent studies revealed that LT synthesis is controlled by the regulation of LT-synthesizing enzymes. We determined the synthesis of LTB4 and LTC4 by specific radioimmunoassay, and the messenger RNA (mRNA) expression of LT-synthesizing enzymes by reverse transcriptase polymerase chain reaction in peripheral polymorphonuclear leukocytes, which were obtained from controls and asthmatic children. The synthesis of LTB4 and LTC4, and the mRNA expression of 5-lipoxygenase, LTA4 hydrolase, and LTC4 synthase were enhanced in the patients. The mRNA expression of LT-synthesizing enzymes was up-regulated, resulting in increased LT synthesis, which may play an important role in the pathogenesis of childhood asthma.
| Abbreviations | ||
| AIAs | = | Aspirin-induced asthma |
| cDNA | = | Complementary DNA |
| cPLA2 | = | Cytosolic phospholipase A2 |
| CRP | = | C-reactive protein |
| DEPC | = | Diethyl pyrocarbonate |
| dNTP | = | Deoxyribonucleoside triphosphate |
| 5-LO | = | Five-lipoxygenase |
| FLAP | = | Five-lipoxygenase activating protein |
| GM-CSF | = | Granulocyte-macrophage colony-stimulating factor |
| HPLC | = | High performance liquid chromatography |
| IL | = | Interleukin |
| LT | = | Leukotriene |
| LTC4 syn | = | Leukotriene C4 synthase |
| mRNA | = | Messenger RNA |
| PBS | = | Phosphate-buffered saline |
| PMNs | = | Polymorphonuclear leukocytes |
| RIA | = | Radioimmunoassay |
| RT-PCR | = | Reverse transcriptase polymerase chain reaction |
| TGF-beta | = | Transforming growth factor-beta |
| WBC | = | White blood cell |