Efficient DMTMM Promoted Nucleoside Loading of CPG Solid Support for Oligonucleotide Synthesis

Abstract

A simple and efficient process for the attachment of protected 2′‐deoxynucleosides to the solid support controlled pore glass (CPG) via succinyl linkage using the mild, non‐carbodiimide dehydrating agent 4‐(4,6‐dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium chloride (DMTMM) has been developed. The protocol is cost effective, does not require anhydrous conditions and is easy to implement.

Keywords:

• 4‐(4,6‐Dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium chloride
• Oligonucleotide
• DNA
• Solid phase synthesis

Acknowledgement

One of the authors MCU is thankful to Isis Pharmaceuticals for the financial assistance and gift of various chemicals and reagents. We also thank Dr. D. L. Cole for his continued support of this project.