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Abstract
Background
This study was designed to evaluate the effects of hydrolyzed protein supplementation upon skeletal muscle total protein and peroxidation in rats following exhaustive swimming exercise.
Methods
Twenty-four rats were randomized to 4 experimental groups (n = 6 per group): control group fed standard diet without exercise (SD), exercise (EX), exercise plus standard diet for 72 hours (EX + SD), and exercise plus standard diet supplemented with hydrolyzed protein (2 g/kg/d) for 72 hours (EX + HP). Immediately following exercise, the EX group was euthanized for collecting plasma and skeletal muscle samples. The EX + SD and EX + HP groups were fed their respective diets for 72 hour still plasma and skeletal muscle collection. Skeletal muscle samples were used to measure levels of total protein (TP), malondialdehyde (MDA), and protein carbonyl (PC). Plasma samples were used to analyze the amino acids spectrum.
Results
Compared with the EX + SD, EX + HP presented the significantly increased TP (P = 0.02) and decreased MDA and PC levels (P = 0.035). MDA was negatively correlated with the methionine levels. Moreover, EX + HP maintained higher levels of plasmaleucine, isoleucine, and methionine than EX + SD, which may be associated with the increased skeletal muscle TP levels observed (P < 0.05).
Conclusions
These results collectively suggest that hydrolyzed protein supplementation can improve skeletal muscle TP and ameliorate peroxidation damage in rats subjected to exhaustive exercise stress, which may be, at least in part, related with the maintenance of plasma leucine, isoleucine, and methionine levels.
Electronic supplementary material
The online version of this article (doi:10.1186/1550-2783-11-5) contains supplementary material, which is available to authorized users.
Xinying Wang, Chenglin Niu contributed equally to this work.
Copyright comment
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
Electronic supplementary material
The online version of this article (doi:10.1186/1550-2783-11-5) contains supplementary material, which is available to authorized users.
Xinying Wang, Chenglin Niu contributed equally to this work.
Copyright comment
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
Funding
This work was supported by National Natural Science Foundation of China (81070282), Natural Science Foundation of Jiangsu Province (BK2010460) and The Six Personnel Peak of Jiangsu Province (079).
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
XW and CN carried out the animal studies and participated in the samples measurement. XW drafted the manuscript. JL performed the statistical analysis and helped to draft the manuscript. NL and JL sconceived of the study, and participated in its design and coordination. All authors read and approved the final manuscript.