Optimising the therapeutic safety of human embryonic stem cells through the evolution of defined culture systems supporting their isolation

Abstract

Realising the therapeutic promise of embryo-derived stem cells is absolutely dependent on the evolution of defined and quality assured in vitro culture systems to mediate their isolation, expansion and delivery. Critical to the safety of these systems, both for the transplant recipient and the general population, is the necessity to eliminate dependence on animal cell-derived reagents to reduce the risk of cross-specific disease transmission. This paper discusses progress in the development of defined culture systems for human embryonic stem cell isolation and the challenges that still must be overcome.

Keywords::

• human embryonic stem cells
• in vitro culture
• regenerative medicine

Acknowledgements

The author at present has no affiliation with any organisation with a financial interest in the subject matter. The hESC lines referenced as derived in the author’s laboratory were funded and are the property of the Geron Corporation, Menlo Park, California, USA. At the time these lines were derived, the author was a paid Geron Corporation consultant.