Bimodal targeting of microsomal cytochrome P450s to mitochondria: implications in drug metabolism and toxicity

Abstract

Importance of the field: Microsomal CYPs are critical for drug metabolism and toxicity. Recent studies show that these CYPs are also present in the mitochondrial compartment of human and rodent tissues. Mitochondrial CYP1A1 and 2E1 show both overlapping and distinct metabolic activities compared to microsomal forms. Mitochondrial CYP2E1 also induces oxidative stress. The mechanisms of mitochondria targeting of CYPs and their role in drug metabolism and toxicity are important factors to consider while determining the drug dose and in drug development.

Areas covered in this review: This review highlights the mechanisms of bimodal targeting of CYP1A1, 2B1, 2E1 and 2D6 to mitochondria and microsomes. The review also discusses differences in structure and function of mitochondrial CYPs.

What the readers will gain: A comprehensive review of the literature on drug metabolism in the mitochondrial compartment and their potential for inducing mitochondrial dysfunction.

Take home message: Studies on the biochemistry, pharmacology and pharmacogenetic analysis of CYPs are mostly focused on the molecular forms associated with the microsomal membrane. However, the mitochondrial CYPs in some individuals can represent a substantial part of the tissue pool and contribute in a significant way to drug metabolism, clearance and toxicity.

Keywords::

• mitochondrial targeting of CYP
• requirement for electron transfer systems
• ROS production and oxidative stress
• structure function of mitochondrial P450

Acknowledgment

We thank HK Anandatheerthavarada from our laboratory for his extensive and important contributions to publications on the mitochondria targeted CYPs.

Notes

This box summarizes key points contained in the article.