Use of phage display technology for the determination of the targets for small-molecule therapeutics

Abstract

Importance of the field: Target discovery of drug-like small-molecules contributes to our understanding of biological phenomena at the molecular level as well as elucidating the mode of action of bioactive compounds. Research in this field is of high value because, in addition to basic observations, the data can be used to directly identify molecular targets or investigate pharmacokinetic characteristics of drugs in clinical use.

Areas covered in this review: In addition to providing a brief overview of phage display (PD) technology, we discuss screening platforms, different types of phage libraries and the application of this method to the determination of targets for small-molecule therapeutics over the past decade.

What the reader will gain: Readers will gain an understanding of the basis of PD technology through successful examples of the use of this method for the determination of targets for small-molecule therapeutics. They will learn what kinds of small-molecules were used to identify their binding partner, what characteristics and drawbacks are present in the use of small-molecule as bait, and what kinds of approaches were introduced in order to improve the technique to overcome the limitations of conventional strategies.

Take home message: A suitable combination of diverse technologies from various different fields can act synergistically to increase throughput and enhance the efficiency of PD technology for the determination of targets for small-molecule therapeutics. The most suitable method for successful target identification of small-molecules of interest using PD technology can often be determined by referring to past examples.

Keywords::

• bioinformatics
• peptide
• phage display
• small-molecule

Acknowledgement

The authors thank S Kobayashi (Department of Medicinal and Life science, Faculty of Pharmaceutical Sciences, Tokyo University of Science) for conducting the organic synthesis of various functional derivatives of compounds under investigation. They also thank K Ohta (Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science) for discussions and conductions concerning phage display and all members of PD research group in Sakaguchi, Sugawara and Kobayashi Lab.

Notes

This box summarizes key points contained in the article.