Transfected cell microarrays: an efficient tool for high-throughput functional analysis

Abstract

Transfected cell microarrays are considered to be a breakthrough methodology for high-throughput and high-content functional genomics. Here, recent advances in the cell microarray field are reviewed, along with its potential to increase the speed of determining gene function. These advances, combined with an increasing number and diversity of gene perturbing systems, such as RNAi and ectopic gene expression, provide tools for expanding our understanding of biology at the systems level.

Keywords::

• automated microscopy
• cDNA
• RNA interference
• transfected cell microarray

Acknowledgements

We thank David Ibberson for carefully reading the manuscript.

Financial disclosure

This work has been supported by the Bundesministerium für Bildung und Forschung with grants 01GR0101 and 01KW0013 and by a grant from the Verbundforschung Baden-Wuerttemberg (Grant No. 24–720.431–1-2/2) to Rainer Pepperkok.