Notch signaling: from stem cell expansion to improving cord blood transplantation

Abstract

Evaluation of: Delaney C, Heimfeld S, Brashem-Stein C, Voorhies H, Manger RL, Bernstein ID. Notch-mediated expansion of human cord blood progenitor cells capable of rapid myeloid reconstitution. Nat. Med. 16, 232–236 (2010).

Ex vivo expansion of hematopoietic stem and progenitor cells has been a major goal for experimental hematologists and stem cell biologists during the last two decades. The clinical implications of such a procedure are obvious, considering the increasing interest in cell therapy protocols. This is particularly true in the setting of cord blood transplants, in which increased numbers of such primitive cells are needed. The study analyzed in this article indicates that by stimulating the Notch signal transduction pathway in primitive cord blood cells it is possible to significantly increase the numbers of both hematopoietic stem and progenitor cells. Furthermore, infusion of such expanded cells in patients receiving a cord blood transplant results in a significant reduction in the time to myeloid engraftment. The relevance of this study is twofold – on the one hand, it shows that the Notch pathway is involved in the expansion capacity of primitive hematopoietic cells in culture, and on the other hand, it indicates that ex vivo-expanded stem/progenitor cells can have a role in hematopoietic transplantation settings.

Keywords::

• cytokines
• Delta1
• ex vivo expansion
• hematopoietic stem/progenitor cells
• Notch

Financial & competing interests disclosure

The author has no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.