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Abstract
Improved protein assays promise to offer new insights into biological processes as well as the identification of new, clinically important biomarkers. In recent years, a number of approaches have been developed where protein-binding reagents, typically antibodies, are equipped with DNA strands to enable protein analyses via powerful nucleic acid detection reactions for improved performance. In this review, we provide a background to this emerging field, and we describe several different ways in which these reagents can improve protein analyses by lowering detection thresholds, improving multiplexing and extending the range of biomolecules available for analysis, both in research settings and in clinical routine.
Acknowledgements
The authors thank Michael J Taussig and Simon Fredriksson for critical reading of this review.
Financial & competing interests disclosure
Work in the authors’ laboratory is supported by funding from the Knut and Alice Wallenberg Foundation, as well as by the Swedish Research Council, Uppsala Berzelii Technology Centre for Neurodiagnostics, the European Community’s Seventh Framework Program (FP7/2007–2013) under grant agreement Health-F4–2008–201418 – READNA and grant agreement 259796 (DiaTools). U Landegren holds stock in Olink Bioscience, having rights to the proximity ligation technique. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.