Association of lncRNA LINC01173 Expression with Vitamin-D and Vitamin B12 Level Among Type 2 Diabetes Patients

Abstract

Background

Type 2 diabetes mellitus (T2DM) has risen to become the world’s most serious public health problem in recent years, and the role of long noncoding RNAs (lncRNAs) in the onset and progression of T2DM, as well as special attention to vitamins, has gotten a lot of attention recently.

Methods

The aim of the study was to analyze lncRNA LINC01173 expression along with assessment of vitamin-D and B12 among the T2DM cases. Quantitative RT-PCR was used to analyze the expression of lncRNA LINC01173. Vitamin-D and B12 were analyzed by chemiluminescence-based assay.

Results

The present study observed that the T2DM cases had 6.67-fold increased lncRNA LINC01173 expression compared to healthy controls. Expression of lncRNA LINC01173 was found to be associated with hypertension (p=0.03), wound healing (p=0.04), and blurred vision (p<0.0001). It was observed that the T2DM cases with vitamin-D deficiency had a significant association with fasting glucose level (p=0.01) and HbA1C level (p=0.01) among the T2DM cases. The association of lncRNA LINC01173 with vitamin-D was analyzed and it was observed that the vitamin-D deficient cases had higher lncRNA LINC01173 expression compared to insufficient T2DM cases (p=0.01) and sufficient T2DM cases (p=0.0006). It was also observed that the T2DM cases with smoking had a 8.33-fold lncRNA LINC01173 expression while non-smokers had a 5.43-fold lncRNA LINC01173 expression (p<0.0001).

Conclusion

The study concluded that the increased lncRNA LINC01173 expression was observed to be linked with alteration in vitamin-D level and smoking habit. Altered expression of lncRNA LINC01173 expression was linked with fasting glucose and HbA1C alteration. Collectively, lncRNA LINC01173 expression, vitamin-D alteration, as well as smoking habit may cause the disease severity and increase the pathogenesis of disease.

Keywords:

• long noncoding RNA
• vitamin-D
• vitamin-B12
• diabetes

Data Sharing Statement

We confirm that the data used during the research will not be shared with anybody/broadcasted in any public domain. The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Ethics and Informed Consent

This research study was ethically approved by the research ethics committee, Ministry of Health, K.S.A., and all participants gave their written informed consent before the study began, and all the ethical principles regarding human experimentation were followed and the study was conducted in accordance with the Declaration of Helsinki.

Acknowledgments

This work is supported by Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia for their financial support through the Small Research Group Project under grant number (RGP.01-115-43). Alanoud Aladel would also like to extend her appreciation to the deanship of scientific research at King Saud University, Riyadh, Saudi Arabia for funding this work through the research chair’s programme.

Disclosure

The authors declare that they have no conflicts of interest.