Tang-Ping-San Decoction Remodel Intestinal Flora and Barrier to Ameliorate Type 2 Diabetes Mellitus in Rodent Model

Abstract

Purpose

Type 2 diabetes mellitus (T2DM) is a complex genetic disease associated with genetic and environmental factors. Previous studies have shown that changes in the gut microbiota may affect the development of host metabolic diseases and promote the progression of T2DM. Tang-ping-san (TPS) decoction can effectively treat T2DM. However, its specific mechanisms must be evaluated.

Patients and Methods

In the present study, we established an animal model of T2DM using a high‑fat diet (HFD) with intraperitoneal injection streptozotocin injection.

Results

The therapeutic effect of TPS decoction on T2DM in mice was initially evaluated. TPS decoction was found to improve hyperglycemia, hyperlipidemia, insulin resistance, and pathological liver, pancreatic, and colon changes. Moreover, it reduced the pro-inflammatory cytokine levels. Based on 16SrRNA sequencing, TPS decoction reduced the Firmicutes/Bacteroidetes ratio at the phylum level. At the genus level, it increased the relative abundances of Akkermansia, Muribaculaceae, and the Eubacterium coprostanoligenes group and decreased the relative abundance of Fusobacterium, Escherichia coli, Dubosiella, and Helicobacter.

Conclusion

TPS decoction improves T2DM and liver function and reduces the risk of hyperglycemia, hyperlipidemia, insulin resistance, pathological organ changes, and inflammatory reactions. The mechanism of TPS decoction in T2DM can be correlated with the reversal of gut microbiota dysfunction and repair of the intestinal mucosal barrier.

Keywords:

• type 2 diabetes mellitus
• tang-ping-san decoction
• gut microbiota
• glycolipid metabolism
• insulin resistance

Ethical Approval

The experimental procedures were reviewed and approved by the Committee for the Care and Use of Laboratory Animals at Yunnan University of Chinese Medicine. (Kunming, Yunnan, China). All procedures were conducted based on the Care and Use of Laboratory Animals published by the US National Institute of Health.

Acknowledgments

This research was supported by National Natural Science Foundation of China (81860646) and a grant from Yunnan Provincial Science and Technology Department (202201AS070084, 202101AZ070001-012, 2019FI016, 202005AC160058, 202105AF150031, YB220309, 30170104891).

Disclosure

The authors report no conflicts of interest in this work