The Correlation Between sP-Selectin and Platelet Count in COVID-19 Patients in Referral Hospital, West Java Indonesia

Abstract

Introduction

sP-selectin is a glycoprotein located in α granules of platelet and endothelial’s Weibel Palade body, as expression to platelet activation and endothelial cell stimulation by SARS-CoV-2 binding with ACE2 receptor. Consumptive thrombocytopenia is also related to platelet activation. Elevation of sP-selectin and thrombocytopenia are related to COVID-19 complication and often correlated with severity of COVID-19.

Purpose

Assess the correlation between sP-selectin and platelet in COVID-19 patients at intensive care and non-intensive care.

Patients and Methods

The study population was hospitalized COVID-19 patients confirmed by Real-Time PCR that underwent platelet examination within 48 hours upon admission, divided into intensive care and non-intensive care group. sP-selectin examination using ELISA methods. Platelet cell count and sP-selectin divided based on normal reference range.

Results

The subjects consist of 24 were in intensive care, 25 were in non-intensive care group. A 66.7% of subject in intensive care group has an elevation in sP-selection (>44.0 ng/mL). Thrombocytopenia was significantly correlated with intensive group (r =−0.32, p<0.05). The combination of platelet count <150.000/mm³ and sP-selectin >44.0ng/mL was not correlated with the intensive and non-intensive group. Platelet and sP-selectin were not correlated with each other.

Conclusion

Thrombocytopenia is able to induce the expression of sP-selectin.

Keywords:

• COVID-19
• sP-selectin
• platelet
• thrombocytopenia

Ethics Approval and Consent to Participate

The institutional review board of Dr. Hasan Sadikin General Hospital Bandung, Indonesia approved the protocol no LB.02.01/X.6.5/337/2022 and all procedures were in compliance with the Helsinki Declaration. All patients gave their informed consent to participate in the study, which was approved by our local Ethics Committee of Dr. Hasan Sadikin General Hospital Bandung, Indonesia.

Acknowledgment

We thank Mrs. Hijrianti for helping us in preanalytic and analytic sample process of this research, Mulya Nurmansyah for statistical analysis, and Verina Logito for helping us in submitting this research.

Disclosure

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.