Neutrophil and Endothelial Cell Membranes Coassembled Roflumilast Nanoparticles Attenuate Myocardial Ischemia/Reperfusion Injury

Abstract

Aim: This study aimed to develop biomimetic nanoparticles (NPs) of roflumilast (ROF) for attenuating myocardial ischemia/reperfusion (MI/R) injury. Materials & methods: We synthesized biomimetic ROF NPs and assembled ROF NPs in neutrophil and endothelial cell membranes (NE/ROF NPs). The physical properties of NE/ROF NPs were characterized and biological functions of NE/ROF NPs were tested in vitro. Targeting characteristics, therapeutic efficacy and safety of NE/ROF NPs were examined in mice model of MI/R. Results: NE/ROF NPs exhibited significant anti-inflammatory and antiadhesion effects. Meanwhile, they was effective in reducing MI/R injury in mice. Furthermore, NE/ROF NPs exhibited stronger targeting capabilities and demonstrated good safety. Conclusion: NE/ROF NPs may be a versatile biomimetic drug-delivery system for attenuating MI/R injury.

Keywords: :

• biomimetic NPs
• endothelial cell
• myocardial ischemia/reperfusion injury
• neutrophil
• roflumilast

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: wwww.tandfonline.com/doi/suppl/10.2217/nnm-2023-0313

Financial disclosure

The present study was funded by the National Key Research and Development Plan 14th Five-Year Nano Frontier Key Special Project (2022YFA1205904), the National Natural Science Foundation of China (82100478), the Jiangsu Planned Projects for Postdoctoral Research Funds (2021K287B), the Natural Science Foundation of Jiangsu Province (BK20200128) and the Nanjing Medical Science and Technology Development Project (YKK21070). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Competing interests disclosure

The authors have no competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

Writing disclosure

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All animal experimental procedures were approved by the Institutional Ethics Committee of Nanjing Drum Tower Hospital (approval no. 2021AE02005).

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.