Abstract
Human secretory endometrium and endometrial cancer tissue both contain acyl-CoA:cholesterol-acyltrans-ferase (ACAT). the optimal assay conditions for measuring ACAT activity in microsomes prepared from these tissues were determined. the activity in normal secretory endometrium was 15.3±1.27 pmol cholesteryl ester formed per mg protein−1 per min−1, whereas 3 out of 4 endometrial cancers had 3–7-fold higher ACAT activity.