The Alteration of Infrastructure and Immunoreactivity of Human Embryonic Organ of Corti Tissue Culture After Exposure to Aminoglycoside (Neomycin) Ototoxicity

Abstract

Mi M-Y, Pao X, Jie T, Yu YJ. the alteration of ultrastructure and immunoreactivity of human embryonic organ of Corti tissue culture after exposure to aminoglycoside (neomycin) ototoxicity. Acta Otolaryngol (Stockh) 1996; 116: 799-804.

Using electron microscopy and GABA immunohistochemistry we evaluated the effects on human embyronic organ of Corti tissue culture of exposure to the ototoxic aminoglycoside antibiotic neomycin at a dose of 1 mM for 48 to 96 hrs. Neomycin induces the formation of multilamellar myeloid structures. These lesions, found only in the basal coil but both. in inner and outer hair cells, were characteristic of the membrane-associated neomycin-induced damage. A large amount i of lipofucsin and numerous lipoid vacuoles as well as vesicle-filled mound-like protrusions were also observed after exposure to neomycin. It seems there is no obvious effect on GABAergic innervation.

Key Words:

• aminoglycoside antibiotic neomycin
• GABA (γ-aminobutyric acid) transmission electron microscopy (TEM)
• human embryonic organ of Corti
• cochlear tissue culture