Effects of Insulin-Like Growth Factor-1 on Expression of Sensory Neuropeptides in Cultured Dorsal Root Ganglion Neurons in the Absence or Presence of Glutamate

ABSTRACT

Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor and plays an important role in promoting axonal growth from dorsal root ganglion (DRG) neurons. Whether IGF-1 could influence expression of sensory neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) of the cultured DRG neurons with excitotoxicity induced by glutamate (Glu) remains unknown. In the present study, primary cultured DRG neurons were used to determine the effects of IGF-1 on expression of SP and CGRP of the neurons with Glu-induced excitotoxicity. The DRG neurons were dissociated and cultured for 48 hr and then exposed to Glu (0.2 mmol/L), IGF-1 (20 nmol/L), and Glu (0.2 mmol/L) plus IGF-1 (20 nmol/L) for additional 12 hr. The DRG neurons were continuously exposed to growth media as control. After that, all the above cultured DRG neurons were processed for detecting SP and CGRP expression by Western blot analysis. The expression of SP and CGRP increased significantly in primary cultured DRG neurons in the presence of IGF-1. The ability of IGF-1 on SP and CGRP expression may play a role in neurogenic inflammation or nociception.

KEYWORDS: :

• calcitinin gene-related peptide
• dorsal root ganglion
• galanin
• glutamate
• insulin-like growth factor-1
• neurotoxicity
• substance P