Abstract
Cytogenetic data has been obtained from 16 primary cultures of non-malignant cervical, uterine, and ovarian tissues. Normal diploid cells constituted the major population in these cultures, with a small tetraploid population.
Forty-eight cultures were set up from biopsies derived from 26 malignant gynaecological tumours. The chromosome constitution of these tumours was also analysed using a direct squash technique. Of the in vitro cultures, 10 cases produced adequate data for comparison with the results from squash preparations. In vitro cultures were characterized by the presence of normal diploid populations in contrast to the marked aneuploidy shown in the squash preparations. In only 1 case was evidence obtained suggesting that malignant cells similar to those present in the in vivo population were present in culture.