Analysis of peptidyl-propyl-cis/trans isomerase 1 (PIN1) gene −842(G > C) and −667(T > C) polymorphic variants in relation to breast cancer risk and clinico-pathological parameters

Abstract

Background. The purpose of this study was to investigate the association between the peptidyl-propyl-cis/trans isomerase 1 (PIN1) −842(G > C) and −667(T > C) polymorphic variants and breast cancer risk among Malaysian ethnic groups namely the Malays, Chinese and Indians, as well as clinico-pathological characteristics of the patients. Patients and Methods. The polymerase chain reaction-restriction fragment length polymorphism was used to genotype 387 breast cancer patients and 252 normal and healthy women who had no history of any malignancy. Results. The distribution of −842(G > C) and −667(T > C) genotypes and alleles frequencies between breast cancer cases and normal individuals showed lack of statistical significance among the Malays (p > 0.05), Chinese (p > 0.05) and Indians (p > 0.05), respectively. Multivariate logistic regression analysis showed that the Malay, Chinese and Indian women who were −842CC homozygotes (p = 0.198, 0.089, 0.620), −842GC heterozygotes (p = 0.492, 0.176, 0.377) and −842C allele carriers (P = 0.226, 0.059, 0.669), respectively, were not associated with breast cancer risk. Furthermore Malay, Chinese and Indian women who were heterozygous (p = 0.777, 0.319, 0.710) and homozygous (p = 0.864, 0.986, 0.954) for −667C allele or carriers of −667C allele (p = 0.977, 0.915, 0.880), respectively, were not associated with an increased risk of breast cancer. None of the −842C and −667C allele genotypes were significantly associated with the clinico-pathological characteristics. Conclusion. Our findings suggest that the polymorphic variants of −842(G > C) and −667(T > C) genes may not appear to have an influence on breast cancer risk among Malaysian Malay, Chinese and Indian women.

Key Words::

• Breast cancer
• PCR-RFLP
• PIN1
• polymorphism
• genotyping

Acknowledgements

This study was financially supported by E-Science Fund grant (02-02-10-SF0016) from the Ministry of Science, Technology and Innovation, Malaysia.

Declaration of interest: The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.