Microanalysis of »Sebum« and Sebum-Like Materials by Temperature Programmed Gas Chromatography

E. Haahti Department of Medical Chemistry, University of Turku, Finland; Baylor University College of Medicine, Houston, Texas

E. C. Horning Department of Medical Chemistry, University of Turku, Finland; Baylor University College of Medicine, Houston, Texas

O. Castrén Department of Medical Chemistry, University of Turku, Finland; Baylor University College of Medicine, Houston, Texas

Abstract

A method employing temperature programmed gas-chromatography for the analysis of complicated lipid mixtures is described.

A direct gas-chromatographic analysis of the diazomethane treated total lipid sample and of the unsaponifiable material gives a general view of the major lipid constituents of the lipid mixture.

The method is applied to the analysis of skin surface fat (“sebum”), cerumen, vernix. caseosa and ovarian dermoid cyst fat.

Records of the analysis are given and are briefly discused.

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Microanalysis of »Sebum« and Sebum-Like Materials by Temperature Programmed Gas Chromatography

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Microanalysis of »Sebum« and Sebum-Like Materials by Temperature Programmed Gas Chromatography

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