Collection and Handling of Bone Marrow Suspensions for Intravenous Use

Abstract

1. The technique for aspiration of human bone marrow for intravenous use, from the posterior iliac spine, is described in detail.

The yield of nucleated cells from a single donor should be not lees than 5–10 × 10⁹, this requiring 200 ml or more to be aspirated.

2. A simple method for removal of fat and filtration of the marrow within a closed-circuit system is developed.

Using bone marrow from rats, there was no significant reduction of the number of megakaryocytes in a suspension which had passed a stainless steel screen with meshes of 105 μm.