Oxygen-Linked Hydrogen Ion Binding of Human Hemoglobin. Effects of Carbon Dioxide and 2,3-Diphosphoglycerate

Abstract

The Bohr coefficient, b, and the Haldane coefficient, h, were measured simultaneously in erythrolysates in the presence and absence of CO₂and 2,3-diphosphoglycerate. We found b—h=—0.04 (—0.023 to —0.054) although b—h=—0 according to the linkage equation. The cause of the observed difference seems to be that his measured as the mean value for oxygen saturation fractions (zHbO²) between 0 and 1, while brefers to a zHbO₂of 0.54. Apparently b(and h) varies with zHbO₂and reaches a numerical maximum at an intermediate zHbO₂value.

Key Words:

• Acid-base equilibrium
• Bohr effect
• carbon dioxide
• 2, 3-diphosphoglycerate
• Haldane effect
• hemoglobin
• hydrogen ion concentration
• oxygen