Radioimmunoassay of β₂-Microglobulin

Abstract

A radioimmunoassay for quantitation of β₂-microglobulin in serum and urine is described. Polyethylene glycol 6000 was used to precipitate the antigen-antibody complex. The assay can be performed within 5 hr. In healthy human subjects (mean age, 30.6 years and 42.0 years for serum and urine determinations, respectively) the mean concentration of β₂-microglobulin in serum was 125 nmo1/1 (range, 49–190 nmo1/1). In men the mean 24-hr urinary excretion was 12.9 nmol (range, 8.6–19.2 nmol), and in women it was 5.5 nmol (range, 2.9–8.7 nmo1). The assay range was 0.23–19.40 nmo1/1, and the detection limit was 0.042 nmo1/1, using a prolonged incubation time. The coefficient of variation based on 20 determinations in serum dilutions with a concentration of β₂-microglobulin of 8.14 and 1.92 nmo1/1 was 8.1 per cent and 9.0 per cent, respectively.

Key Words:

• β₂-microglobulin
• polyethylene glycol precipitation
• radioimmunoassay