Cationic Proteins of Human Granulocytes V. Interaction with Plasma Protease Inhibitors

P. Venge Dept. of Clinical Chemistry, University Hospital, Uppsala, Sweden; Dept. of Internal Medicine, Research Laboratory for Clinical Hematology, University of Lund, Lund, Sweden

I. Olsson Dept. of Clinical Chemistry, University Hospital, Uppsala, Sweden; Dept. of Internal Medicine, Research Laboratory for Clinical Hematology, University of Lund, Lund, Sweden

H. Odeberg Dept. of Clinical Chemistry, University Hospital, Uppsala, Sweden; Dept. of Internal Medicine, Research Laboratory for Clinical Hematology, University of Lund, Lund, Sweden

Abstract

Several cationic proteins of human granulocytes possess chymotrypsin-like and bactericidal activities. The heat-labile chymotrypsin-like activity is inhibited by serum, owing to complex formation wiui α₂-macroglobulin and α₁-antitrypsin. The molar affinity of the cationic proteins for α₂-macroglobu-lin is much higher than that for α₁-antitrypsin. The results indicate that the molar combining ratios are 1:1 for cationic protein to α₁-antitrypsin and 2:1 for cationic protein to α₂-macroglobulin. The proteolytic activity against fibrinogen and casein is inhibited by both α₂-macroglobulin and α₁-antitrypsin, whereas the activity against small molecular synthetic substrates is inhibited by α₁-antitrypsin but not α₂-macroglobulin. The heat-stable bactericidal action of the cationic proteins against Staphylococcus was also inhibited by serum, probably owing to complex formation with α₂-macroglobulin and α₁-antitrypsin.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Cationic Proteins of Human Granulocytes V. Interaction with Plasma Protease Inhibitors

Information for

Open access

Opportunities

Help and information

Cationic Proteins of Human Granulocytes V. Interaction with Plasma Protease Inhibitors

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature