High-density lipoprotein cholesterol assay by magnesium dextransulphate precipitation

Abstract

A method for determination of high-density lipoprotein cholesterol (HDL-C) is evaluated: after precipitation of low and very low lipoprotein cholesterol in serum by magnesium dextransulphate, HDL-C is determined by an enzymatic method. The precision of the method was good: coefficient of variation 4%. The accuracy was good, evaluated by correlating HDL-C results from the present assay to the results obtained from three other HDL-C assays (n=17): ultracentrifugation r=0.91, phosphotungstate/MgCl₂ r=0.98, and α-lipoprotein determination by electrophoresis r=0.91 (p<0.01). Repeated analysis showed that serum may be kept at 4 °C for 1 month, at -20 °C for 2 months, and requires -80 °C for longer storage. Ten patients with acute myocardial infarction showed significantly lower HDL-C from day 4 in the acute phase and during the first 3 months follow up (p<0·05). Eighty patients with peripheral vascular disease, who were compared to a group of matched controls, also showed significantly decreased serum HDL-C (p<0·01). The present HDL-C assay is easy, fast and reliable and is considered a valuable clinical test.

Key Words:

• acute myocardial infarction
• lipoprotein electrophoresis
• peripheral vascular disease
• ultracentrifugation