Measurements on the forward light scattering intensities of monodisperse latex particles and sphered or unsphered red blood cells

Abstract

Characteristics with respect to light scattering intensity (LSI) were measured on latex spheres and on sphered and unsphered red blood cells (RBC). From the discrepancies in LSI signals from polymer spheres and RBCs it is obvious that latices cannot be used for absolute calibration of RBC sizes. Sphering of RBCs did not give rise to a closer resemblance. Monodisperse latex spheres produce extremely narrow size distribution histograms. Regarding these sharp peaks and the long-term stability, polymer spheres offer definite advantages if compared with blood controls to which preservatives were added. A shift from the RBC size distribution histogram, caused by gradually developing instrument drift, can be easily detected and corrected at an early stage.

Key Words:

• laser haemocytometry
• light scattering
• polystyrene spheres
• quality control
• size distribution histogram
• sphering