Enhancement of fibrinolytic activity of human plasma in the presence of acetone

Abstract

The fibrinolytic system was studied in normal human plasma containing increasing concentrations of acetone up to 23.4mmoU^-'. Fibrinolytic activity measured as euglobulin clot lysis time [ECLT] and amidase activities toward chromogenic peptide substrates H-D-Valyl-L-Leucyl-L-Lysine-p-nitroanilide 2 HC1 [S-2251], designed for plasmin determination, H-D-Valyl-L-Phenylalanyl-L-Lysine-p-nitroanilide 2 HC1 [S-2390], designed for the determination of t-PA in plasma via plasminogen activation and H-D-Prolyl-L-Phenyl-Alanyl-L-Argi-nine-p-nitro-anilide 2 HC1 [S-2302], designed for the determination of kallikrein and activated Hageman factor, increased when 15.7mmoll⁻¹ concentration of acetone was reached. A parallel increase of esterolytic [substrate: naphthol-AS-acetate] activity was observed in euglobulin fractions. Crossed Immunoelectrophoresis [CIE] revealed changes in fibrinogen profiles of plasma enriched with acetone as compared to native plasma. These findings suggest that acetone present in plasma in concentrations comparable to those found in some pathological states might activate fibrinolytic system.

Key Words:

• amidolytic activity
• chromogenic peptide substrates
• esterolytic activity