Enzyme Activities in Jejunal Biopsy Samples from Patients with Adult Coeliac Disease with and without Steatorrhoea

Abstract

Highly sensitive techniques have been used for the assay of a range of marker enzymes including lactase, sucrase, alkaline phosphatase, leucyl-β-naphthylamidase (brush border), and 5′-nucleotidase (basolateral membrane) in jejunal biopsy homogenates from patients with adult coeliac disease with and without steatorrhoea and from a control group. The absorption of D-xylose and vitamin B₁₂ was compared in the two groups with coeliac disease. All enzymes assayed were equally depressed in both groups of coeliac disease as compared with the controls. The absorption of D-xylose and vitamin B₁₂ were reduced in the patients with steatorrhoea compared with those without steatorrhoea. The findings suggest that lack of steatorrhoea in some patients with coeliac disease is due to better preservation of the ileal function rather than to a less severe jejunal mucosal injury.

Key-words:

• Biopsy
• coeliac disease
• enzymes
• jejunal mucosa
• steatorrhoea