Immunocytochemical Studies of Gastric H⁺,K⁺-ATPase in the Developing Rat

H. F. Helander Center for Ulcer Research and Education, VA Wadsworth Hospital Center, Los Angeles, California, USA

D. Anderson Center for Ulcer Research and Education, VA Wadsworth Hospital Center, Los Angeles, California, USA

K. G. Helander Center for Ulcer Research and Education, VA Wadsworth Hospital Center, Los Angeles, California, USA

A. Smolka Center for Ulcer Research and Education, VA Wadsworth Hospital Center, Los Angeles, California, USA

G. Sachs Center for Ulcer Research and Education, VA Wadsworth Hospital Center, Los Angeles, California, USA

Abstract

The appearance of the enzyme H⁺,K⁺-ATPase was studied in the gastric mucosa of rats during the perinatal period. By means of monoclonal antibodies against the enzyme, immunoreactivity was regularly detected in parietal cells 1 day before birth. The intensity of the staining and the frequency of stained cells increased up to 10-12 days after birth, when adult levels were approached. Electron microscopy showed that the initial staining occurred at the apical surface of the parietal cells, with only faint or no staining at the secretory canaliculi. From 5 days after birth immunoreactivity was observed also at the tubulovesicular membranes.

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Immunocytochemical Studies of Gastric H⁺,K⁺-ATPase in the Developing Rat

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Immunocytochemical Studies of Gastric H+,K+-ATPase in the Developing Rat

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Immunocytochemical Studies of Gastric H⁺,K⁺-ATPase in the Developing Rat