Alteration of Gastroduodenal Acetylcholine Metabolism Induced by 2-Deoxy-D-Glucose in Rats

Abstract

The effect of 2-Deoxy-D-glucose (2-DG) on acetylcholine (ACh) metabolism in the stomach and duodenum of rats was studied. 2-DG significantly increased [³H] choline uptake, [³H]ACh synthesis, and choline acetyltransferase and acetylcholinesterase activities in parallel with an increase in gastric acid secretion. The conversion rate of uptaken [³H] choline to [³H] ACh, the turnover rate of ACh, and the contents of ACh and choline were negligibly influenced by 2-DG. 2-Buten-4-olide, which inhibits vagus nerve activity via the central nervous system, prevented the 2-DG increased [³H] choline uptake and subsequent [³H]ACh synthesis as well as the increase in gastric acid secretion. These results suggest that neuronal activity of the vagus nerve is coupled with [³H] choline uptake and subsequent [³H] ACh synthesis, and that cholinergic neuronal activity depends on quantitative changes in ACh metabolism in the gastroduodenum.

Key Words:

• ACh metabolism
• choline uptake
• 2-Deoxy-D-glucose
• gastroduodenum
• vagus nerve