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Original Article

Evaluation of Simple Tests for Detection of HIV Antibodies: Analysis of Interobserver Variation in Tanzania

, , , , , , , , , & show all
Pages 283-285 | Published online: 08 Jul 2009
 

Abstract

200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

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