A 90-day intravenous administration toxicity study of CaO-SiO₂-P₂O₅-B₂O₃ glass-ceramics (BGS-7) in rat

Abstract

CaO-SiO₂-P₂O₅-B₂O₃ glass ceramics directly bond to bone and have potential use as a bone substitute material. The present study evaluated the toxicity from subchronic intravenous administration of CaO-SiO₂-P₂O₅-B₂O₃ glass ceramics to male and female Sprague-Dawley rats. The rats were divided into four groups, each consisting of 10 male and 10 female rats, and administered different amounts of CaO-SiO₂-P₂O₅-B₂O₃ glass ceramics (aqueous extract 5, 2.5, and 1.25 mL/kg body weight/day and saline) 7 days per week for 90 consecutive days. During the experiment, no deaths were observed in any groups, and there were no remarkable changes in clinical signs, body weight, food and water intake, hematological and serum biochemical parameters, organ weight, and histopathological findings between the control and treated groups. The results show no adverse toxic effects of CaO-SiO₂-P₂O₅-B₂O₃ glass ceramics (aqueous extract 5 mL/kg body weight/day) to rats of either sex.

Keywords::

• CaO-SiO₂-P₂O₅-B₂O₃ glass-ceramics
• intravenous subchronic toxicity
• rat
• bone graft extender

Acknowledgements

This work was supported by the Common Core Technology Development Program of the Korean Ministry of Knowledge and Economy (#A00-A01-3302-02-1-3). The authors would like to express their gratitude to the Clinical Research Institute of Seoul National University Hospital for executing animal tests and assessment and also to Bioalpha (Seongnam, Korea) for providing CaO-SiO₂-P₂O₅-B₂O₃ glass-ceramics (BGS-7) used in this study.

Declaration of interest: All authors disclose here that there are no conflicts of interest that could inappropriately have influenced the outcome of the present study.