Techniques for the Detection of Leukocytospermia in Human Semen

Abstract

During the cytological evaluation of 1200 spermiocytograms, we used prestained microscope slides (Testsimplets) in 200 cases as a screening method. In 50 cases, a check was made to determine whether leukocytospermia could be detected with the aid of Cytur-Test. The findings were compared with smears that were routinely stained by the Papanicolaou technique and subsequently differentiated. The cytological examination of human sperm with the aid of Testsimplets provides a technically simple and rapid procedure that has sufficient informative value to permit the diagnosis or exclusion of the leukocytospermia and the sperm morphology. Leukocytospermia could also be detected by the use of Cytur-Test. The presence of larger numbers of spermatogenic cells does not lead to a blue coloration of the Cytur-Test areas. In cases of leukocytospermia there is a clear blue coloration. These two rapid techniques are useful for the diagnosis of leukocytospermia.

Key Words:

• Male fertility
• Leukocytospermia
• Staining techniques
• Cytur-Test
• Testsimplets.