Size Heterogeneity of Immunoreactive Prolactin in Stored Semen and Seminal Plasma

M. L. Smith The Department of Clinical Investigation and Endocrine Service, Madigan Army Medical Center, Tacoma, WA, 98431, USA

W. A. Luqman The Department of Clinical Investigation and Endocrine Service, Madigan Army Medical Center, Tacoma, WA, 98431, USA

B. L. Fariss The Department of Clinical Investigation and Endocrine Service, Madigan Army Medical Center, Tacoma, WA, 98431, USA

Abstract

The difference between semen and seminal plasma immunoreactive human prolactin (iPRL) correlates with sperm count. Seminal plasma separated from sperm before freezing (SPB), after freezing (SPA), and serum were fractionated by G100 Sephadex chromatography. SPA contained four different molecular weight fractions of iPRL. Each fraction contained approximately 25% of the total iPRL measured. SPB and serum each had three molecular weight fractions and most of the iPRL coeluted with monomeric ¹²⁵I-hPRL.

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Size Heterogeneity of Immunoreactive Prolactin in Stored Semen and Seminal Plasma

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Size Heterogeneity of Immunoreactive Prolactin in Stored Semen and Seminal Plasma

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