Purification of Human Testosterone-Estradiol Binding Globulin (TeBG): A Specific RIA of Human TeBG

Abstract

The immunological properties of human serum testosterone-estradiol binding globulin (TeBG) which was purified by affinity chromatography and hydroxyapatite column chromatography were investigated and a specific radioimmunoassay (RIA) for human TeBG was established. The purified TeBG here obtained was 86,000 in molecular weight and the antibody prepared against this TeBG slightly cross-reacted with human immunoglobulin G (IgG). The human TeBG labeled with ¹²⁵I had a high binding activity to the antihuman TeBG antibody, and the sensitivity of the RIA thus obtained was enough to detect the TeBG in 2 μl of the serum.

Key Words:

• TeB
• Purificatio
• RI
• Testosterone