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Abstract
Sialic acid was estimated both chemically in populations of heads and tails of spermatozoa from normospermic human seminal fluid and by transmission electron microscopy, using a specific labeling technique. The results of both procedures show that sialic acid is located exclusively in the head plasma membrane and in the acrosomal membranes. It is suggested that localization of sialic acid close to that reported for neuraminidase in sperm might be of importance in the fertilization process.