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Abstract
Acridine orange fluorescence, semen analysis, and zona-free hamster egg penetration tests were performed on 100 consecutive semen samples. No significant correlation was detected between sperm motility and red fluorescence. However, abnormal sperm morphology correlated significantly with red fluorescence (p ± 0.001) and fluorescence index (p ± 0.001). Presence of a high percentage of red fluorescing sperm did not prevent their penetration of zona-free hamster egg, indicating that the presence of abnormal DNA had no effect on the sperm fertility. The significance of acridine orange fluorescence male fertility test requires further elucidation.