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Abstract
Two semen specimens from 34 fertile donors and 82 male partners of infertile couples were evaluated for semen quality. Sperm morphology was evaluated with unstained specimens and following Papanico-laou staining. A significant difference (p < 0.001) was found in the percentage of morphologically normal spermatozoa in stained and unstained semen smears. Fertile semen donors had a significantly (p < 0.001) higher percentage of normal sperm than men of unproven fertility. When compared to the fertile donors, 78% of the infertile men had increased teratospermia. Papanicolaou staining appears to enhance sperm morphology and allow improved discrimination of sperm head abnormalities.