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Abstract
A cryosyringe and floating platform were developed to simplify the procedures for human sperm cyropreservation without sacrificing post-thaw sperm quality. The cryosyringe was developed as a vessel for cryopreservation and as a device for post-thaw insemination. A platform-like polystyrene foam board 3-cm in height was floated on the surface of liquid nitrogen in a polystyrene foam freezing container. The cryosyringe containing sperm/KS-II medium mixture was placed on it for 5 min, then it was dropped into the liquid nitrogen. Freezing velocity was -27d`C/min. Poor quality semen specimens (40 ± 14 × 106/ml, 18 ± 2.6% motility, n = 9) were concentrated (87 ± 46 × 106/nl, 29 ± 9.1%) previously and then cryopreserved in the cryosyringe or in a commercial tuberculin syringe under the conditions established in the present study. The post-thaw sperm motility of the specimens in the cryosyringe (18 ± 7.4%) was significantly superior (p < 0.05) to that in the commercial tuberculin syringe (13 ± 5.3% motility).