Trypsin-Like Arginine Amidases Including Plasminogen and Plasmin in Human Seminal Plasma by Affinity Adsorption and Elution

T. Kobayashi Department of Obstetrics and Gynecology, School of Medicine, Keio University, Shinanomachi, Shinjiku-ku, Tokyo, 156

Y. Matsuda First Department of Biochemistry, Meiji College of Pharmacy, Nozawa, Setagaya-ku, Tokyo, 154

J. Y. Park Department of Obstetrics and Gynecology, School of Medicine, Keio University, Shinanomachi, Shinjiku-ku, Tokyo, 156

I. Hara Department of Obstetrics and Gynecology, School of Medicine, Keio University, Shinanomachi, Shinjiku-ku, Tokyo, 156

S. Kaneko Department of Obstetrics and Gynecology, School of Medicine, Keio University, Shinanomachi, Shinjiku-ku, Tokyo, 156

Y. Fujimoto Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Otaru-shi, Hokkaido, 047-02, Japan

S. Nozawa Department of Obstetrics and Gynecology, School of Medicine, Keio University, Shinanomachi, Shinjiku-ku, Tokyo, 156

S. Akihama First Department of Biochemistry, Meiji College of Pharmacy, Nozawa, Setagaya-ku, Tokyo, 154

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Abstract

An enzyme preparation with affinity to a lysine column was detected from a DEAE-cellulose-adsorbed preparation of human seminal plasma containing plasminogen and plasmin. Two kinds of trypsin-like acidic arginine amidase activity with different affinity to lima bean trypsin inhibitor (LBTI) and aprotinin affinity column were detected from the DEAE-cellulose-adsorbed preparation after treatment of the lysine column. Two kinds of trypsin-like basic arginine amidase activity were also separated by the above-mentioned affinity adsorptions from a CM-cellulose-adsorbed preparation of human seminal plasma. The effect of calcium chloride on these two enzymes was different from human acrosin.

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Trypsin-Like Arginine Amidases Including Plasminogen and Plasmin in Human Seminal Plasma by Affinity Adsorption and Elution

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Trypsin-Like Arginine Amidases Including Plasminogen and Plasmin in Human Seminal Plasma by Affinity Adsorption and Elution

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