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Abstract
The serum concentrations of FSH, LH, prolactin, testosterone, and estradiol and the enzymatic activities of hyaluronidase, glucosidases (α-glucosidase, β-glucosidase, α-mannosidase, N-acetyl-β-D-glucosaminidase, β-glucuronidase, and β-galactosidase), lactate dehydrogenase and its isoenzyrnes (LDH1, LDH2, LDH3, LDH-X, LDH4), and total proteins were measured in the semen of 69 subjects (8 normozoospermic controls, 7 secretory, and 54 excretory azoospermic subjects). FSH levels rose with the deterioration in spermatogenesis and served to differentiate the secretory from the excretory azoospermias. The only source of hyaluronidase and LDH-X in the ejaculate is the spermatozoa, α-Glucosidase activity essentially originates in the epididymis. The seminal determination of α-glucosidase and, to a lesser extent, α-mannosidase and N-acetyl-β-D-glucosaminidase helps rapidly, sensitively, reliably, and noninvasively to differentiate secretory azoospermias (with higher enzymatic activity) from the excretory type (less enzymatic activity) and may be of use in identifying with a certain degree of reliability the site of obstruction in the male genital tract.