Ultrastructural Parameters of Fertile Cryopreserved Human Sperm

E. E. Oettlé Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Tygerberg Hospital, Parow, South Africa; Andrology Laboratory, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, Observatory, Cape Town, South Africa

U. A. Clarke Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Tygerberg Hospital, Parow, South Africa; Andrology Laboratory, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, Observatory, Cape Town, South Africa

J. McLoughlin Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Tygerberg Hospital, Parow, South Africa; Andrology Laboratory, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, Observatory, Cape Town, South Africa

M. R. Levin Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Tygerberg Hospital, Parow, South Africa; Andrology Laboratory, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, Observatory, Cape Town, South Africa

K. Wiswedel Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Tygerberg Hospital, Parow, South Africa; Andrology Laboratory, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, Observatory, Cape Town, South Africa

T. F. Kruger Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Tygerberg Hospital, Parow, South Africa; Andrology Laboratory, Department of Obstetrics and Gynaecology, Groote Schuur Hospital, Observatory, Cape Town, South Africa

Abstract

Two hundred and forty-four cryopreserved semen samples were used for artificial insemination by donor (AID). All samples were examined for motility and concentration. Thirty of the samples resulted in pregnancies. These samples were further examined ultrastructurally. There was no difference in sperm motility or concentration between the samples that did or did not result in a pregnancy. The ultrastructural characteristics of the samples that resulted in pregnancies revealed that only 15% of sperm (SD = 7.67) possessed normal morphology and had undamaged acrosomes after cryopreserva-tion.

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Ultrastructural Parameters of Fertile Cryopreserved Human Sperm

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Ultrastructural Parameters of Fertile Cryopreserved Human Sperm

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