ICAM-1 targeted catalase encapsulated PLGA-b-PEG nanoparticles against vascular oxidative stress

Abstract

Targeted delivery of therapeutics is the favourable idea, whereas it is possible to distribute the therapeutically active drug molecule only to the site of action. For this purpose, in this study, catalase encapsulated poly(d,l-lactide-co-glycolide)-block-poly(ethylene glycol) (PLGA-b-PEG) nanoparticles were developed and an endothelial target molecule (anti-ICAM-1) was conjugated to this carrier system in order to decrease the oxidative stress level in the target site. According to the enzymatic activity results, initial catalase activity of nanoparticles was increased from 27.39 U/mg to up to 45.66 U/mg by adding 5 mg/mL bovine serum albumin (BSA). After 4 h, initial catalase activity was preserved up to 46.98% while free catalase retained less than 4% of its activity in proteolytic environment. Furthermore, FITC labelled anti-ICAM-1 targeted catalase encapsulated nanoparticles (anti-ICAM-1/CatNPs) were rapidly taken up by cultured endothelial cells and concomitantly endothelial cells were resistant to H₂O₂ induced oxidative impairment.

• Catalase
• ICAM-1
• oxidative stress
• PLGA-b-PEG nanoparticle
• vascular targeting

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

This study was financially supported by Hacettepe University, Scientific Research Projects Coordination Unit (Grant No. 010A301001).