Effects of vitreous from photocoagulated eyes on retinal micro vascular cells in culture: a preliminary report

Mike Boulton Department of Clinical Ophthalmology, Institute of Ophthalmology, Judd Street, London, WCIH 9QS

Carol Lane Department of Clinical Ophthalmology, Institute of Ophthalmology, Judd Street, London, WCIH 9QS

Arvind Singh Department of Clinical Ophthalmology, Institute of Ophthalmology, Judd Street, London, WCIH 9QS

Hong Chai Wong Surgical Vitreoretinal Unit, Moorfields Eye Hospital, City Road, London, EC1, UK

Ann Bridgman Department of Clinical Ophthalmology, Institute of Ophthalmology, Judd Street, London, WCIH 9QS

David Mcleod Surgical Vitreoretinal Unit, Moorfields Eye Hospital, City Road, London, EC1, UK

John Marshall Department of Clinical Ophthalmology, Institute of Ophthalmology, Judd Street, London, WCIH 9QS

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Abstract

Panretinal photocoagulatlon was carried out in mini pigs in an attempt to elucidate the mechanisms involved in laser therapy. The effect of the vitreous from these eyes on the proliferation of retinal microvascular endothelial cells was then studied. Vitreous removed 4 days after panretinal photocoagulation had no effect on the proliferation of bovine retinal microvascular endothelial cells in basal medium but inhibited proliferation in growth medium. Control vitreous was mitogenic for the microvascular cells in basal medium but this effect was not observed in growth medium.

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Effects of vitreous from photocoagulated eyes on retinal micro vascular cells in culture: a preliminary report

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Effects of vitreous from photocoagulated eyes on retinal micro vascular cells in culture: a preliminary report

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