Guanosine 5′-0-thiotriphosphate and NaF stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis in bovine corneal epithelium

Abstract

The role of a GTP-binding protein in activation of phospholipase C in bovine corneal epithelium was determined by investigating the effects of non-hydrolyzable GTP analog, GTP-γ-S, and NaF on breakdown of phosphatidylinositol 4,5-bisphosphate (PIP₂) in this tissue. GTP-γ-S (2–50 μM), when introduced into the permeabilized corneal epithelial cells labeled with myo-[³H]inositol, dose-dependently increased the formation of myoinositol trisphosphate (IP₃). Other guanine nucleotides and ATP were ineffective. Incubation of ³²P-prelabeled corneal epithelium with NaF (2–50 mM) resulted in increased breakdown of PIP₂ and increased synthesis of phosphatidic acid. In myo-[³H]inositol-labeled tissue, NaF dose-dependently increased the accumulation of IP₃. Microsomal membrane fraction from corneal epithelium was found to contain phospholipase C activity towards endogenous phosphatidylinositol 4-phosphate and PIP₂. The enzyme activity was stimulated by Ca²⁺ (100μM). Addition of GTP-γ-S to microsomal fraction containing phosphoinosi-tides which were radiolabeled with ³²Pi in situ or with [γ-³²P]ATP in vitro caused a dose dependent hydrolysis of PIP₂. These data, taken collectively, suggest that a GTP-binding protein is involved in activation of phospholipase C towards PIP₂ in bovine corneal epithelium, and that this guanine nucleotide regulatory protein may serve to couple norepinephrine and 5-hydroxytryptamine receptors to phospholipase C during transmembrane signalling.