Biochemical Modulation by 5-Fluorouracil and 1-Folinic Acid of Tumor Uptake of Intra-Arterial 5-[¹²³I]Iodo-2'-Deoxyuridine in Patients with Liver Metastases from Colorectal Cancer

Abstract

In previous studies we demonstrated a high tumor-targeting value of the ¹²³I-labeled thymidine analogue 5-iodo-2'-deoxyuridine (IUdR) infused intra-arterially in patients with liver metastases from colorectal cancer. In the present study we have explored the possibility of enhancing tumor uptake of [¹²³I]IUdR, by biochemical modulation with 5-fluorouracil (5-FU) and 1-folinic acid (FA), a drug combination known to inhibit thymidylate synthetase in tumor cells. The investigation was carried out employing diagnostic imaging doses of [¹²³I]IUdR, much lower than possible therapeutic levels. In the baseline study, [¹²³I]IUdR was infused into the hepatic artery of patients with inoperable liver metastases from colorectal cancer, and a second infusion was performed one week later, after intra-arterial administration of 5-FU and FA. The effect was evaluated by comparing tumor uptake of [¹²³I]IUdR in the second study with that of the baseline study. The average tumor uptake immediately after [¹²³I]IUdR infusion was 9.1% ID in the baseline study, increasing to 14.9% ID after pretreatment with 5-FU and FA. The average enhancement in early tumor uptake of [¹²³I]IUdR induced by biochemical modulation was 72%. This enhancement was sustained at 18 and 42 hours after infusion (stable uptake). The results encourage the pretreatment of patients with 5-FU and FA prior to radioiodinated IUdR administration and suggest its inclusion in therapeutic protocols employing IUdR labeled with ¹²³I or ¹²⁵I as a source of highly cytotoxic Auger electrons.